Fig. 4: Liver gene expression in LXRα W441F mice. | Nature Communications

Fig. 4: Liver gene expression in LXRα W441F mice.

From: A mutation in LXRα uncovers a role for cholesterol sensing in limiting metabolic dysfunction-associated steatohepatitis

Fig. 4

RNA-Seq was carried out as described in the Methods section using liver RNA isolated from 11–12 week old male control (W/W), heterozygous mutant (W/F), and homozygous mutant (F/F) W441F mice maintained on normal chow diet (n = 4 group). a PCA analysis. b Summary of enriched GO terms in pairwise comparisons. Blue = downregulated, red = upregulated. Adjusted p values were determined using Fisher exact test. c–h Gene expression measured by real-time PCR as described in the Methods section. *Statistically significant difference between control and mutant mice determined by one-way ANOVA (p ≤ 0.05; n = 6 W/W/sex, n = 6 W/F/sex. n = 6 F/F females, n = 4 F/F males). Boxes extend from the 25th–75th percentiles. The center line in the middle of the boxes represents the median. Whiskers extend from the lowest to the highest point. i Cd68 (top) and Clec4f (bottom) staining of representative liver sections from 11–12 week old female control (W/W), heterozygous mutant (W/F), and homozygous mutant (F/F) W441F mice maintained on normal chow diet. Source data are provided as Source Data file.

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