Fig. 5: Fibrosis and liver damage in LXRα W441F mice.

Female and male 11–12 week old control (W/W), heterozygous mutant (W/F), and homozygous mutant (F/F) W441F mice were fed the MASH diet. A subset of mice was sacrificed after 2 weeks. The remaining mice were maintained on the MASH diet for an additional 2 weeks and treated with vehicle or 10 mg/kg T0901317 daily by oral gavage as described in the Methods section. a Liver sections from representative female mice stained with Picrosirius Red to visualize collagen. b, c Quantification of the % surface area positive for Picrosirius Red in liver sections from female and male mice as described in the Methods section. *Statistically significant difference between control and mutant mice in the same treatment group; **statistically significant difference between vehicle and T0901317 treated mice of the same genotype determined by two-way ANOVA (p ≤ 0.05, n = 5/group). d–g Plasma ALT and AST levels. *Statistically significant difference between control and mutant mice in the same treatment group; **statistically significant difference between vehicle and T0901317 treated mice of the same genotype determined by two-way ANOVA (p ≤ 0.05). 2 weeks: n = 12 W/W/sex, n = 18 W/F/sex, n = 12 F/F females and 13 F/F males. 4 weeks + vehicle: n = 6 W/W/sex, n = 9 W/F/sex, n = 6 F/F/sex. 4 weeks + T0901317: n = 6 W/W/sex, n = 9 W/F/sex, n = 6 F/F females and 7 F/F males. Boxes extend from the 25th–75th percentiles. The center line in the middle of the boxes represents the median. Whiskers extend from the lowest to the highest point. Source data are provided as Source Data file.