Fig. 5: Cell type specific CREs have significant physical (HiChIP) interaction with the MYC promoter. | Nature Communications

Fig. 5: Cell type specific CREs have significant physical (HiChIP) interaction with the MYC promoter.

From: Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancers

Fig. 5

a Schematic of H3K27ac HiChIP workflow. b (left) H3K27ac HiChIP contacts at 20 kb resolution between MYC and other elements in the TAD. Highlighted in color are the significant CREs identified in each CRISPRi screen and their HiChIP contacts with MYC. (right) The percent of CRISPRi-identified CREs that contact MYC (first column) and the percent of total contacts with MYC that include CRISPRi CREs (second column). c Pearson correlation of H3K27ac HiChIP from A549 cells with histone modifications. d Recall analysis of gRNA sequences within CREs by the indicated biochemical signature. Plot represents an average across all cell lines. e Overlap of H3K27ac HiChIP and CRISPRi perturbation screens for A549 (lung), PC-9 (lung) and HT-29 (colon). These three cell lines share a CRE (dotted box) at the promoter of CCAT1. The single dotted line indicates MYC. Source data are provided as a Source Data file.

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