Fig. 4: Modulating rice tiller numbers by the TCD system in rice.
a Prediction of OsTB1 as an intrinsically disordered protein (IDP) using PLAAC and D2P2 algorithms indicated by Prion domain (PrD)-like score and disorder score, respectively. The bottom section displays low complexity regions and the RING domain as annotated by the SMART website. b, c Pilot experiments to show the degradation of OsTB1 by the OsTB1–E3TCD1 degrader through microscopic observation (b) and immunoblot analysis (c). Ponceaus S staining was used as a control for protein loading. For microscopic observation, 35S::CFP serves as the control. Semi-quantitative RT-PCR is conducted against YFP with NbUBQ as the internal control. d, e The rice tiller numbers in rice Zhonghua11 (ZH11) plants transformed with 35S::E3TCD1 or 35S::OsTB1–E3TCD1. Two independent transgenic lines (#1 and #2) were used for each construct. The bars show the mean ± s.d. (n = 30) of tiller numbers, and a two-sided Student’s t test was used to determine the significance. f Quantitative RT-PCR to show the relative endogenous OsTB1 mRNA levels in the transgenic lines. Primers binding to the untranslated region, which was not included in the OsTB1–E3TCD1 transgene, were used to amplify the endogenous OsTB1 gene. The bars show the mean ± s.d. (n = 3) after normalization to ZH11, a two-sided Student’s t test was used to determine the significance. Scale bar, 10 µm (b), 10 cm (d). Source data are provided as a Source Data file.
