Fig. 5: Modulating flowering times in Arabidopsis and rice with the TCD system. | Nature Communications

Fig. 5: Modulating flowering times in Arabidopsis and rice with the TCD system.

From: Genetically-encoded targeted protein degradation technology to remove endogenous condensation-prone proteins and improve crop performance

Fig. 5

See also Supplementary Figs. 6–9. a, b The flowering phenotype of Arabidopsis Col-0 plants transformed with 35S::E3TCD1 or 35S::AtELF3–E3TCD1. Two independent transgenic lines (#1 and #2) are shown. The bars show the mean ± s.d. (n  =  16) of the days to flowering after seeding, and a two-sided Student’s t test was used to determine the significance. c, d The flowering phenotype of rice Zhonghua11 (ZH11) plants transformed with 35S::E3TCD1, 35S::OsELF3-1–E3TCD1 or 35S::OsELF3-2–E3TCD1. Two independent transgenic lines (#1 and #2) were used for each construct. The bars show the mean ± s.d. (n  =  15) of the days to heading after seeding under the natural long-day (LD) condition, and a two-sided Student’s t test was used to determine the significance. e–g In planta degradation assay to demonstrate the degradation of the OsELF3-1 or OsELF3-2 target protein by the OsELF3-1–E3TCD1 or OsELF3-2–E3TCD1 degrader by microscopic observation (e) and immunoblot analysis (f, OsELF3-1; g, OsELF3-2). The plasmids of 35S::CFP/35S::OsELF3-1–YFP or 35S::CFP/35S::OsELF3-2–YFP were co-transformed into the protoplasts prepared from the transgenic plants. Coomassie brilliant blue (CBB) was used as a control for protein loading. Semi-quantitative RT-PCR was conducted against YFP with NbUBQ as the internal control. Scale bar, 3 cm (a), 10 cm (c), 10 µm (e). Source data are provided as a Source Data file.

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