Fig. 6: Conditional TCD with the TBF1 expression control cassette. | Nature Communications

Fig. 6: Conditional TCD with the TBF1 expression control cassette.

From: Genetically-encoded targeted protein degradation technology to remove endogenous condensation-prone proteins and improve crop performance

Fig. 6

See also Supplementary Fig. 8. a, b Symptoms and quantification after the fungal pathogen M. oryzae infection in the growth chamber on rice Zhonghua11 (ZH11) plants transformed with 35S::E3TCD1, 35S::OsELF3-1–E3TCD1, 35S::OsELF3-2–E3TCD1 or ProTBF1-uORFsTBF1::OsELF3-2–E3TCD1. Two independent transgenic lines (#1 and #2) were used for each construct. ProTBF1-uORFsTBF1, the ‘TBF1-cassette’ consisting of the immune-inducible promoter (ProTBF1) and two pathogen-responsive upstream open reading frames (uORFsTBF1) of the Arabidopsis TBF1 gene. The bars show the mean ± s.d. (n  =  5) of the lesion length, and a two-sided Student’s t test was used to determine the significance. c, d The flowering phenotype of rice Zhonghua11 (ZH11) plants transformed with ProTBF1-uORFsTBF1::OsELF3-2–E3TCD1. Two independent transgenic lines (#1 and #2) were used for each construct. The bars show the mean ± s.d. (n  =  15) of the days to heading after seeding under the natural long-day (LD) condition, and a two-sided Student’s t test was used to determine the significance. e The phylogenetic tree to show the homologs of E3TCD1 in Arabidopsis thaliana (At), Oryza sativa (Os), Zea mays (Zm), Triticum aestivum (Ta), Brassica napus (Bn), and Solanum lycopersicum (Sl). Scale bar, 1 cm (a), 10 cm (c). Source data are provided as a Source Data file.

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