Fig. 1: Human LUC7 proteins impact 5′ splice sites with distinct composition.
From: LUC7 proteins define two major classes of 5′ splice sites in animals and plants
A Sequence logos of annotated 5′SS in yeast (top) and humans (bottom). B Cryo-EM data of yeast Luc7p contacting U1 snRNA:5′SS duplex from Bai et al., 20188. C Representative sequence logos of 5′SS for all cassette exons (left) and exons with significantly increased or decreased inclusion upon depletion of LUC7L2 or LUC7L3. D Schematic of 5′SS Balance score, which is defined as the number of consensus bases at positions +4/ + 5/ + 6 minus the number of consensus bases at –3/–2/–1 of a 5′SS. E Mean phyloP score for human 5′SS of each possible 5′SS Balance score. F Log-odds estimates of 5′SS differential inclusion versus skipping as a function of the 5′SS Balance score for each human LUC7 RNA-seq dataset. Point estimates are derived from 100 bootstrap samples, each containing 500 differentially included (FDR < 0.1, dPSI > 0) and 500 differentially skipped (FDR < 0.1, dPSI <0) events, with replacement. Error bars represent the 95% confidence intervals, calculated as the standard deviation of the 100 bootstrap estimates. Source data are provided as a Source Data file.
