Fig. 4: LUC7 specificity for subclasses of 5′SS depends on the structured N-terminal region.
From: LUC7 proteins define two major classes of 5′ splice sites in animals and plants
A eCLIP enrichment of LUC7L2 around windows of constitutive and cassette exon splice sites, with crosslinks aggregated into 10 nt bins (top). Pearson correlation between LUC7 score and LUC7L2 eCLIP enrichment is shown, with significant correlations (p < 0.05) identified by Pearson’s correlation test. B Protein domain structure of human LUC7 proteins and experimentally investigated chimeric proteins. C Change (“delta”) in percent spliced in (qRT-PCR) from transfected minigenes containing different internal exons of introns following overexpression of LUC7 WT or chimeric cDNA (shown at right). Mean is plotted with error bars representing standard error of the mean, and bars are color-coded by the Balance score of the internal exon’s 5′SS. D Heat map of Pearson correlations of delta percent spliced values for each LUC7 WT or chimeric cDNA overexpressed in (C). Source data are provided as a Source Data file.
