Fig. 3: Morphological detection of Arabidopsis ex-proteasomes by transmission electron microscopy (TEM) of the APFp. | Nature Communications

Fig. 3: Morphological detection of Arabidopsis ex-proteasomes by transmission electron microscopy (TEM) of the APFp.

From: Proteasomes accumulate in the plant apoplast where they participate in microbe-associated molecular pattern (MAMP)-triggered pathogen defense

Fig. 3

The resuspended APFp was visualized by negative-stain TEM after enrichment from wild-type leaves. a A micrograph at low magnification showing EVs7. Bottom panels show close-up views of representative EVs. b A higher magnification view of the APFp identifying particles with the characteristic CP and RP-CP 26S proteasome architectures. The boxes highlight an end-on view of a CP barrel (arrows identifying the signature heptameric α-subunit ring) with its axial pore, and a side view of a CP singly capped with an RP. Orange arrowheads locate other possible top views of CP barrels. c Representative top and side views of individual CP particles with their 4-tiered αββα-subunit barrel assembly, side views of singly-capped CP-RP particles, and a rare doubly-capped RP-CP-RP 26S particle. The bottom right image shows a 2D class average for the side view of the CP barrel generated from 40 EM images. Scales bars = 8 nm.

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