Fig. 3: LDHB is essential for optimal mouse pDC IFN-I production in vitro and in vivo.

a Relative expression of LDHB was evaluated by RT-qPCR in purified cDC2, cDC1, and pDC from the spleens of uninfected and LCMV Cl13 infected mice at day 9 p.i. b ELISA quantification of IFNα secreted by pDC isolated from the spleen (left) or bone marrow (right) of WT or LDHB^−/− mice after 12 h stimulation with CpG-A. c ELISA (left) or qPCR (right) quantification of secreted IFNα or Ifna transcript in pDC isolated from Flt3L cultures derived from WT or LDHB^−/− mice after 12 h stimulation with CpG-A. d ELISA quantification of IFNα in the serum of WT (black) or LDHB^−/− (red) mice treated with CpG-A. b–d Unstimulated pDC were below the limit of detection. e Experimental design for f-g, 50:50 mixed bone marrow chimeras for BDCA2-DTR:WT or BDCA2-DTR:LDHB were generated, then treated with DT daily from two days prior to infection to deplete BDCA2 expressing pDCs. Mice were then infected with MHV, 48 h.p.i pDCs were sorted and virus titers were quantified. f Expression of Ifna transcript in pDC from e. g Plaque assay quantification of MHV in livers from e. Data are pooled from 2 (d), 3 (c right, g), 4 (c, left), or 5 (b) independent experiments or representative of 2 (a) or 3 (f) independent experiments. Data are shown as mean ± SEM. Statistics used are One way ANOVA with Tukey Correction (a), Two tailed Student’s t-test (b, c, f, g)¸ multiple unpaired two tailed t-tests with two stage step up FDR (d).