Fig. 5: m⁶A demethylases FTO and ALKBH5 were both increased in alveolar bone tissues of AP rats and OC, respectively.

a The total m⁶A methylation level in the alveolar bone (AB) tissues of rats with AP was measured (n = 3 independent experiments; mean ± SD). b The total m⁶A methylation level was measured (n = 3 independent experiments; mean ± SD). c Immunoblotting of obesity-associated protein (FTO) and Alk B homologous protein 5 (ALKBH5) expression in the AB tissues of rats with AP. The expression levels of FTO and ALKBH5 were determined (n = 3 independent experiments with 3 technical replicates; mean ± SD). d FTO mRNA levels of and ALKBH5 gene in the AB tissues of rats with AP were determined (n = 3 independent experiments; mean ± SD). e Immunoblotting of FTO and ALKBH5 expression during osteoclastogenesis. The expression level of FTO and ALKBH5 protein during osteoclastogenesis were determined (n = 3 independent experiments; mean ± SD). f FTO and ALKBH5 mRNA levels during osteoclastogenesis were determined (n = 3 independent experiments; mean ± SD). g Representative images of phalloidin (green) and FTO (red) detection by immunofluorescence in bone marrow macrophage (BMDM)-derived osteoclast (OC) at 5 days (n = 3 independent experiments). h Representative images of FTO (green) and nuclear factor-κB receptor activator (RANK) (red) detection by immunofluorescence in the AB tissues of rats with AP at 6 weeks and in the control group (n = 3 independent experiments). Fiji software was used to assessed the intensity of colocalization. Fluorescence intensity profiles of positive cells showed different colocalized signals of endogenous FTO and RANK. For the AP rat model, the contralateral teeth served as control. The major statistical procedures applied were: Shapro–Wiik test (a–g), F-test (a–f), Repeated measures ANOVA and Dunnett-t test (a–f), student-t test (g). The statistical test used was two-sided. Source data are available online for this figure.