Fig. 7: Targeting FTO by AAV9-shFTO-GFP and Dac51 both alleviated the ABR in the rat AP model. | Nature Communications

Fig. 7: Targeting FTO by AAV9-shFTO-GFP and Dac51 both alleviated the ABR in the rat AP model.

From: FTO-associated osteoclastogenesis promotes alveolar bone resorption in apical periodontitis male rat via the HK1/USP14/RANK pathway

Fig. 7

a Flow diagram of the treatment of apical periodontitis (AP) with the macrophage-targeted AAV9-shFTO-GFP (6.67 × 1011 v.g/rat, 10 μL/rat) or Dac51(2 mg/kg) in rats, respectively (n = 9 rats per group). b The representative images of obesity-associated protein (FTO) (red) and nuclear factor-κB receptor activator (RANK) (pink) detection in the alveolar bone (AB) tissues of rats (n = 3 independent experiments). c The representative micro-CT images of AB tissues in the rats (n = 3 independent experiments; mean ± SD). The bone resorption fraction was defined as bone resorption surface area (BRS)/bone resorption volume (BRV). d The representative micro-CT images of AB tissues in the rats with AP (n = 3 independent experiments; mean ± SD). e Morphometric imaging showed TRAP staining in the AB tissues of rats with AP (n = 3 independent experiments with 3 technical replicates; mean ± SD). Arrowheads pointed the TRAP-positive surfaces. TRAP-positive multinucleated cells were counted as OC. f Morphometric imaging showed TRAP staining in the AB tissues of rats with AP (n = 3 independent experiments with 3 technical replicates; mean ± SD). g The total m6A methylation level in the AB tissues of rats (n = 3 independent experiments; mean ± SD). h The total m6A methylation level in the AB tissues of rats was measured (n = 3 independent experiments; mean ± SD). i The expression level of the relevant protein in the AB tissues of rats were determined (n = 3 independent experiments; mean ± SD). j The relevant protein level of in the AB tissues of rats with AP were determined (n = 3 independent experiments; mean ± SD). The AAV9-shNC group AP rats were received the treatment of unedited plasmid carried by AAV9 vector; the PBS group AP rats were treated with PBS, a solvent for Dac51. The major statistical procedures applied were: Shapro–Wiik test (c–j), F-test (j), Student-t test (c–h), Wilcoxon test (i), Kruskal–Wallis H test and Dunnett-t test (j). The statistical test used was two-sided. Source data are available online for this figure.

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