Fig. 8: Demethylase FTO mediated m6A demethylation modification of HK1.
a m6A enrichment on hexokinase 1 (HK1) mRNAs in the alveolar bone (AB) tissues of rats. The IgG antibody was served as a negative control (n = 3 independent experiments; mean ± SD). b The interplay between obesity-associated protein (FTO) and HK1 was measured in the AB tissues of rats. The IgG antibody was served as a negative control (n = 3 independent experiments; mean ± SD). c The interplay between FTO and HK1 was measured in bone marrow macrophage (BMDM)-derived osteoclast (OC). The IgG antibody was served as a negative control (n = 3 independent experiments; mean ± SD). d The relative protein levels in 293T cells were determined (n = 3 independent experiments; mean ± SD). e The relative protein levels of HK1 in OC were determined (n = 3 independent experiments; mean ± SD). f The HK1 protein levels in OC after treatment with Dac51 were determined (n = 3 independent experiments; mean ± SD). g The mRNA stability of HK1 after FTO overexpression in 293 T cells was detected (n = 3 independent experiments with 3 technical replicates; mean ± SD). h Measuring the levels of glycolysis in OC (n = 3 independent experiments with 3 technical replicates; mean ± SD). i The levels of the mitochondrial activity in OC by oxygen consumption rate (OCR) (n = 3 independent experiments with 3 technical replicates; mean ± SD). j The levels of glycolysis in OC after Dac51 treatment by extracellular acidification rate (ECAR) (n = 3 independent experiments with 3 technical replicates; mean ± SD). For the AP rat model, the contralateral teeth served as control. The Lenti-shNC group BMDM cells were received the treatment of unedited plasmid carried by lentiviral vector. The major statistical procedures applied were: Shapro–Wiik test (a–j), F-test (b, d, e, f, h, j), paired t test (a), Student-t test (c, g, i), One-way ANOVA and Dunnett-t test (d, e, f, h, j). The statistical test used was two-sided. Source data are available online for this figure.
