Fig. 2: Deficiency of hepatic PDI results in hypolipidemia and hepatosteatosis.

a, b Plasma TG (a) and cholesterol (b) levels of Alb-cre/Pdi^fl/fl (n = 6 for WT, n = 6 for KO), Pdia2^−/− (n = 3 for WT, n = 3 for KO), Alb-cre/Pdia3^fl/fl (n = 7 for WT, n = 6 for KO), Pdia4^−/− (n = 5 for WT, n = 4 for KO), Pdia5^−/− (n = 4 for WT, n = 4 for KO), Pdia8^−/− (n = 3 for WT, n = 3 for KO), Pdia9^−/− (n = 3 for WT, n = 3 for KO), Pdia11^−/− (n = 7 for WT, n = 5 for KO), Pdia13^−/− (n = 6 for WT, n = 4 for KO), Pdia14^−/− (n = 8 for WT, n = 8 for KO), Pdia15^−/− (n = 4 for WT, n = 3 for KO), CAG-cre/Pdia16^fl/fl (n = 3 for WT, n = 3 for KO), Pdia19^−/− (n = 5 for WT, n = 6 for KO) mice, and corresponding control mice were shown as indicated. c, d Representative gross appearance of the livers (c) and the ratio of liver weight to body weight (d) of the Alb-cre/Pdi^fl/fl and Pdi^fl/fl mice are shown (male, n = 5 for the WT and n = 3 for the KO). e–g The levels of hepatic TG (n = 3 biological replicates in each group) (e), CHOL (n = 3 biological replicates in each group) (f), and free fatty acid (n = 4 biological replicates in each group) (g) were measured using the assay kits according to the manufacturer’s instructions. h, i Representative micrographs of liver sections stained with H&E (Scale bars = 50 μm) (h) and Oil Red O (Scale bars = 100 μm) (i) (n = 3 biological replicates in each group) are shown. Two-tailed Student’s t test were used. Data are presented as mean ± SEM. Source data are provided as a Source Data file.