Fig. 1: MAGTs from different plant species display diverse acceptor recognition. | Nature Communications

Fig. 1: MAGTs from different plant species display diverse acceptor recognition.

From: Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls

Fig. 1

a Model structure of β-mannans modified with Gal side chains, which are found in different plant species. A single-letter nomenclature for the residues in the backbone and possible side chains are indicated. b An MAGT clade of GT34 phylogenetic tree. Amino acid sequences of MAGTs in different plant species were determined from the GT34 phylogenetic tree in Supplementary Fig. 1. AtXXT1 was selected as an outgroup. Highlighted MAGTs were characterised in the present study. Plant species were as followed: Pab, Picea abies; Pme, Pseudotsuga menziesii; Pta, Pinus taeda; Amt, Amborella trichopoda; At, Arabidopsis thaliana; Br, Brassica rapa; Ct, Cyamopsis tetragonoloba; Gm, Glycine max; Md, Malus domestica; Ptr, Populus trichocarpa; Solyc, Solanum lycopersicum; Bd, Brachypodium distachyon; Hv, Hordeum vulgare; Os, Oryza sativa; Sb, Sorghum bicolor; Spipo, Spirodela polyrhiza; Zosma, Zostera marina. Scale bar represents 0.5 substitutions per site. In vitro activity of MAGTs towards homomannan (c), glucomannan (d), and patterned glucomannan (e). Two independent attempts yielded comparable results. The presence of Gal on the products was confirmed by α-galactosidase (α-Galase) treatment. S, standard of Man and mannooligosaccharides with D.P. 2-6. Structures of oligosaccharides in (d) were determined individually by further analysis shown in Supplementary Fig. 3. Source data are provided as a Source Data file.

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