Fig. 3: Engineering of AcGGM in Arabidopsis secondary cell walls by MAGTs. | Nature Communications

Fig. 3: Engineering of AcGGM in Arabidopsis secondary cell walls by MAGTs.

From: Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls

Fig. 3

a Gal/Man ratio of AcGGM in pIRX3::MAGT lines determined from the ratio of AnGH5-digested products. Data are mean values and standard deviation of three biological replicates. One-way ANOVA (two-tailed) indicated a significant effect of genotype on mucilage area (p = 1.21 \(\times\) 10−5; F8,18 = 11.41). Results of post hoc multiple comparisons (Dunnet’s method compared with wild-type) are indicated by asterisks (*p < 0.05; **p < 0.01) with p values. Digestion profile and the measured proportion of products were shown in Supplementary Fig. 7. b Galactosylated oligosaccharide released from AcGGM in pIRX3::MAGTs expressing Arabidopsis. Unique bands found in pIRX3::MAGTs lines (I-IV) were further analysed (Supplementary Fig. 8). c Summary of galactosylated oligosaccharides found in the regions I-IV. Dotted squares indicate oligosaccharides with clustered galactosylation. Source data are provided as a Source Data file.

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