Fig. 4: Galactosylation changes the interaction of AcGGM with cellulose.
a AnGH5-digestion profile of hot water extract from bottom stem AIR of pIRX3::MAGT lines. The presence of products indicates the high extractability of AcGGM. S, standards of Man and mannooligosaccharides with D.P. 2-6. b Monosaccharides composition of the hot water extract. Data are mean values and standard deviation of three biological replicates. The significant difference was determined by one-way ANOVA on each monosaccharide, followed by Dunnets multiple comparisons where pRIX3::MAGT lines were compared with wild-type (*p < 0.05; **p < 0.01). F values and P values were provided in Supplementary Data 6. c In vitro cellulose-binding isotherms. KOH extracts of pRIX3::MAGT lines of a range of concentrations were incubated with and without Avicel. The amount of Man in the supernatant was determined by HPAEC-PAD, from which the bound Man was calculated. Data from three biological replicates were plotted in the graphs. Each point indicates a mean value with SEM of three technical replicates. Non-linear regression was used to fit the curves. The area with the shaded colour indicates the 95% confidence interval. Bmax and Kd were calculated from the fitted curves and shown in the graphs with SEM. Source data are provided as a Source Data file.
