Fig. 6: Summary of substrate specificity of MAGT and effect of galactosylation on AcGGM-cellulose interaction. | Nature Communications

Fig. 6: Summary of substrate specificity of MAGT and effect of galactosylation on AcGGM-cellulose interaction.

From: Glucomannan engineering highlights roles of galactosyl modification in fine-tuning cellulose-glucomannan interaction in Arabidopsis cell walls

Fig. 6

a MAGTs characterised here showed different acceptor recognition. Circles indicate the substrates that MAGTs can recognise, while Crosses are substrates that MAGTs cannot act on. 1) AtMAGT1/2 and PtMAGT are specific to glucomannan while CtMAGT has a promiscuous activity towards any (gluco)mannan. 2) PtMAGT prefers Glc residues on the acceptor at subsite 3, and shows no activity on the patterned glucomannan. 3) AtMAGT2 has the additional pocket for Gal adducts, which allows it to transfer Gal near Gal branches on the acceptor. b Low galactosylation does not affect the interaction of AcGGM with cellulose in muro whereas high galactosylation ends up incomplete binding to cellulose presumably due to the loss of proper conformation to bind to cellulose and a steric hindrance of the high number of branches.

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