Fig. 7: THZ1 inhibits ANLN-Pol II clusters and ESCC cell proliferation.

A KYSE150 cells were treated with 0.1 μM THZ1 for 3 h, ANLN (pink) and Pol II (green) clusters were observed with super-resolution imaging. Scale bar, 3 μm. B, C Effect of THZ1 on the number of different types of clusters. n = 42 (Control); n = 45 (THZ1). The error bars indicate the mean ± SDs. Two-tailed Student’s t tests were performed to determine the significance. D Relative fluorescence intensity of protein signals per cell per focal plane. n = 42 (Control); n = 45 (THZ1). The error bars indicate the mean ± SDs. Two-tailed Student’s t tests were performed to determine the significance. E, F Heatmap (E) and box plots (F) showing expression changes of all protein-coding genes (Total, n = 11376), super-enhancer-associated genes (SE, n = 652), and target genes (TG, n = 84) with or without THZ1 treatment. The error bars indicate the mean ± SDs. Two-tailed Student’s t tests were performed to determine the significance. G KYSE150 cells were treated with 0.1 μM THZ1, the mRNA expression of the indicated target genes was measured. The error bars indicate the mean ± SDs. Two-tailed Student’s t tests were performed to determine the significance. H The indicated cell lines were treated with different concentrations of THZ1 for 48 h, and cell viability was subsequently analyzed by MTS assay. The error bars indicate the mean ± SDs. I ESCC cell lines were treated with low toxicity THZ1 (0.04 μM) or/and F806 (20 μM) for 48 h, and cell viability was measured by MTS assay. n = 3 for each group. The error bars indicate the mean ± SDs. The data are representative of at least three independent experiments with similar results. Source data are provided as a Source Data file.