Fig. 1: Heterogeneity in the requirements of different CDKs and cyclins.

A Heat map depicting the distinct clustering of multiple tumor cell lines based on their dependencies to the indicated CDKs and cyclins. The dependency was measured based on the CHRONOS scores from DEPMAP dataset. B The enrichment of different tumor types within each cluster. C Violin plots illustrating the distribution of cell lines from cluster 3 and cluster 2 based on their dependencies to indicated genes. D Live cell imaging to monitor the proliferation of the indicated cell lines following RNAi-mediated depletion of CDK4 and CDK2. Representative cell lines were selected from clusters 2, 3 and 6. Error bars indicate mean and SD from n = 3 technical replicates. The experiments were done as 3 biological replicates. *** represents p value < 0.0001 as determined by 2-way ANOVA comparing siCDK4 and siCDK2. E Biochemical analysis to interrogate the impact of CDK4 and CDK2 depletion on cell cycle proteins on the indicated cell lines. F Differential effect of CCNE1 and CCNA2 depletion on the proliferation of indicated cell lines. Error bars were determined based on mean and SD from n = 4 technical replicates. The experiments were repeated 3 independent times. *** indicates p value < 0.0001 as determined by 2-way ANOVA, comparing CCNE1 and CCNA2 depletion. G Cell cycle analysis on MCF7, MB157 and 3226 cell lines following CCNE1 and CCNA2 depletion. The cell population at each phase was quantified and graphed. Error bars represent mean and SD from n = 3 biological replicates. H Bivariate flow cytometry analysis to determine the BrdU incorporation in MB157 and MCF7 cells following the CCNA2 depletion. I Western blotting on MB157, KURAMOCHI and MCF7 following the deletion of CCNE1 and CCNA2 to determine the effect on cell cycle proteins. Western blotting was done at two independent times (E & I). Source data are provided as a source data file.