Fig. 9: In vivo anti-tumor efficacy of INX-315 in combination with palbociclib. | Nature Communications

Fig. 9: In vivo anti-tumor efficacy of INX-315 in combination with palbociclib.

From: Discrete vulnerability to pharmacological CDK2 inhibition is governed by heterogeneity of the cancer cell cycle

Fig. 9

A Relative tumor growth on MCF7 xenografts treated with vehicle (n = 4) and INX-315 (200 mg/Kg) (n = 5). B Change in mice body weight that were treated with vehicle (n = 3) and INX-315 (n = 5). Error bars represent mean and SEM for (A & B). *** represents p value < 0.0001 (A), p value = 0.5564 (B) as determined by 2-way ANOVA comparing vehicle- and INX-315-treated groups. C Column graph depicting the tumor weights from MCF7 xenografts that were treated with vehicle (n = 3 mice) and INX-315 (n = 4 mice). Error bars represent mean and SEM. Statistical significance was determined by two-tailed unpaired student t-test. (*** p < 0.0001) comparing vehicle- and INX-315-treated groups. D Representative images of the H&E staining on the tissues from MCF7 xenografts that were treated with vehicle and INX-315. Scale bar represents 50 microns. E Relative tumor growth on MIA PaCa-2 xenografts and two different PDAC derived PDX, 3226 and 1222 to determine the effect of INX-315 in combination with palbociclib. The treatment groups for 3226 PDX are vehicle (n = 5), Palbo (n = 4), INX-315 (n = 5) and Palbo/INX-315 (n = 6). The treatment groups for MiaPaCa-2 xenografts are vehicle (n = 5), Palbo (n = 4), INX-315 (n = 3) and Palbo/INX-315 (n = 5). The treatment groups for 1222 PDX are vehicle (n = 7), Palbo (n = 3), INX-315 (n = 3) and Palbo/INX-315 (n = 5). Error bars represent mean and SEM. *** represents p value < 0.0001 (3226 and MiaPaCa-2), * p value = 0.0225 (1222 PDX) as determined by 2-way ANOVA, comparing the INX-315 and Palbo/INX-315-treated groups. F Representative images of the H&E staining and immunohistochemical staining of pRB on the 1222 and 3226 PDX tissues that were treated with vehicle, palbociclib, INX-315 and Palbo/INX-315 groups. The staining was done on tissues derived from n = 3 mice for each condition. The scale bar represents 50 microns. G Representative images of MIF staining on the indicated proteins from the 1222 PDX that were treated with vehicle and INX-315 in combination with palbociclib. The scale bar represents 50 microns (H) Column graphs depicting the fraction of tumor cells expressing the indicated proteins from the vehicle (2 technical replicates, n = 3 mice) -and Palbo/INX-315-treated groups (2 technical replicates, n = 3 mice). Error bars were determined from mean and SEM. * represents p value 0.0457 (pRB (S807/811)), *** indicates p value < 0.0001 (KI67) and ** represents p value 0.0087 (MCM2) as determined by two-tailed unpaired student t-test. All the comparisons were made between the vehicle- and Palbo/INX-315 treated groups. Source data are provided as a source data file.

Back to article page