Fig. 7: The BBIR-T cell therapy could inhibit fibrosis expansion and improve cardiac remodeling. | Nature Communications

Fig. 7: The BBIR-T cell therapy could inhibit fibrosis expansion and improve cardiac remodeling.

From: CD248-targeted BBIR-T cell therapy against late-activated fibroblasts in cardiac repair after myocardial infarction

Fig. 7

a Flow cytometry of fibroblasts isolated from hearts of C57BL/6 J WT and Pdgfra-CRE:tdTomato mouses. Typical images show CD248+ F-Act versus the pdgfrα+ fibroblasts ratio. Quantifying the pdgfrα+ fibroblast ratio in live cells and the CD248+ F-Act ratio in pdgfrα+ fibroblasts. n = 7 mice in the MI + BF + BBIR-T group and n = 5 mice in other groups. b Representative full-view images of heart HE and Picro-Sirius Red staining, magnifying the PIZ. Scale bars = 2.5 mm and 100 µm. Quantifying LV wall thickness (mm), infarct size (mm2) and PIZ size (mm2). Calculating the ratio of infarct size or PIZ size to LV area. n = 7 mice of each group. c Serum BNP levels were detected by ELISA. The number of mice (n) in each group was labelled in the bottom of the bar chart. d–f HW/BW ratio, HW/TL ratio and lung wet/dry weight ratio were shown. The number of mice in each group was labelled in the bottom of the bar charts. g Double staining of fluorescent WGA-FITC and DAPI. Scale bar = 50 μm. Quantify each group’s cardiomyocyte CSA (μm2). n = 5 mice of each group. a–g Data are mean ± SEM and p-values are displayed in the bar charts, one-way ANOVA followed by Tukey’s multiple comparison test. Source data are provided as a Source Data file.

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