Fig. 1: Quantifying protein leakage artifacts.
From: Limiting the impact of protein leakage in single-cell proteomics
a Images of cells in the CellenONE nozzle taken with brightfield and with the green fluorescent channel. The two cells shown are not clearly distinguishable from the brightfield but one cell is permeable and thus positive for the Sytox green fluorescent dye. b The UMAP dimensionality reduction shows co-clustering of cells from fresh and frozen batches that were recorded as permeable via Sytox green. c Percentages of permeable cells for the two sample handling conditions are shown in the dot plot. d Differences in protein fold changes between permeable and intact cells for all proteins. e Differences in protein fold changes between permeable and intact cells are categorized by subcellular compartment.
