Fig. 4: cPlk1i does not interfere with cell cycle progression.

a Cell cycle profiles of 4 of the conditions shown in b of HeLa cells after 7 h of treatment with the free inhibitor Plk1i and the caged inhibitor cPlk1i (- and + uncaging). The cell cycle profile of the remaining conditions plotted in b are shown in Supplementary Fig. 5. b Plot of G2/M content of the cell cycle profiles after treatment of HeLa cells for 7 h with the indicated inhibitors. Graph shows mean ± standard error of the mean (SEM). Ordinary one-way ANOVA was performed comparing each condition to the DMSO control. N = 3, with 20’000 cells per condition per experiment. Each dot represents the percentage of one experiment. c and d Microscopy picture showing fluorescence from cPlk1i (1 µM) (top) and SiR-tubulin (25 nM) for reference (bottom) (c) and quantifications (d). Shown is the mean intensity ± standard deviation (SD), each dot represents average intensity of all cells analyzed per image, n = 272, N = 1, 6 technical replicates. Ordinary one-way ANOVA was performed. For all panels no label or ns means not significant: p > 0.8 (exact p values are provided in the Source Data file), **: p = 0.002, ****: p < 0.0001. Source data are provided as a Source Data file.