Fig. 5: Automated evolution of pCNF-RS.
From: Integrating protein language models and automatic biofoundry for enhanced protein evolution
a Mutation sites selected for constructing combinatorial mutagenesis with PLMeAE Module II, shown in the crystal structure (PDB:1J1U) of MjTyrRS. b Sequence alignment of the variants obtained in four rounds of directed evolution. c Fitness values of variants obtained in four rounds of directed evolution. The activities of variants were tested in three independent repetitions. 91, 90, 94 and 96 variants are tested in first, second, third and fourth round, respectively. The violin plots show the distribution of fitness values. A mini box-and-whisker diagram is included, where the whiskers represent the minimum and maximum values, the box spans from the first to the third quartile, and the central spot marks the median value. Source data are provided as a Source Data file. d Location of variants tested in fourth round evolution using PLMeAE Module I.
