Fig. 5: The anti-obesity treatment and long-term benefit with MN-MP/aUCP1 patch under US irradiation.

a Schematic illustration for different treatments of mice. b Changes in body weight of mice at different treatment conditions. Data are presented as means ± SD (n = 5 mice per group). c Total intake of HFD in each group of mice under different treatment conditions (n = 5 mice per group). d Representative obese mice photographs on the 21st day of various treatments as indicated. e Representative photographs of various isolated adipose tissues extracted from mice in (d). f Weight of the extracted adipose tissue. Data are presented as means ± SD (n = 5 biologically independent samples). g Representative H&E images from different parts and different groups of adipose tissues (Scale bar: 50 µm). h Corresponding morphometric analysis of the adipocyte size. Data are presented as means ± SD (n = 3 biologically independent samples). i H&E and oil red O stained observation of liver sections isolated from mice after 21 days of different treatments (Scale bar: 50 µm). j Daily diet intake of different treated mice during the 15-day weight recovery monitoring segment. Data are presented as means ± SD (n = 3 mice per group). Change of body weight (k) and the corresponding percentage of weight change (l) during the following 15 days after treatment at various conditions. Data are presented as means ± SD (n = 3 mice per group). The experiments for g and i were repeated three times independently with similar results. Statistical differences for b, f, h, k, and l were calculated using two-tailed unpaired student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Source data are provided as a Source Data file.