Fig. 8: Mechanistic exploration of the weight-loss capacity of MN-MP/aUCP1 + US. | Nature Communications

Fig. 8: Mechanistic exploration of the weight-loss capacity of MN-MP/aUCP1 + US.

From: Transdermal microneedle-assisted ultrasound-enhanced CRISPRa system to enable sono-gene therapy for obesity

Fig. 8

Cluster analysis (a), volcano map (b) and venn diagram (c) of DEGs in RNAseq between untreated and MN-MP/aUCP1 + US treated IgWAT 21 days post treatment (n = 5 biologically independent samples). (P < 0.05, |fold change | ≥2). Statistical difference was calculated using Negative binomial distribution test. d Significant enrichment in GO terms (top 30, n = 5 biologically independent samples). e, f GSEA on RNAseq data of IgWAT isolated from untreated and MN-MP/aUCP1 + US treated mice (n = 5 biologically independent samples). g Heatmap of DEGs associated with glucose or fatty acid metabolism, mitochondria, and lipogenesis and differentiation (n = 5 biologically independent samples). Statistical differences for d, e, and f were calculated using Fisher’s exact test.

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