Fig. 2: EEF-2 T56 phosphorylation is unchanged in starving C. elegans. | Nature Communications

Fig. 2: EEF-2 T56 phosphorylation is unchanged in starving C. elegans.

From: Eukaryotic Elongation Factor 2 Kinase EFK-1/eEF2K promotes starvation resistance by preventing oxidative damage in C. elegans

Fig. 2

A, B WB of p-EEF-2, EEF-2, tubulin, and eIF2α Ser51 phosphorylation (p-eIF2α) in WT and efk-1 mutants under the following conditions: A fed and 8-h starved in L4 stage larvae, and B overnight hatched (0 d), 4-days starved (4 d) and 9-days starved (9 d) at L1 stage. N = 4; p-EEF-2 and p-eIF2α quantified in Fig. S2A, B, D, E; for full membrane see Fig. S2C, F. C WB of p-EEF-2, EEF-2, and tubulin in WT L4 larvae either fed or under acute starvation for 5 min (5 m), 30 min (30 m), 1 h, and 2 h. N = 3; for whole membrane images, see Fig. S2H. D WB of p-EEF-2, EEF-2 and tubulin in overnight hatched and various fed conditions in L1-stage WT worms. Worms were either hatched without food, hatched in food, or hatched without food and refed for 8 h. N = 3; p-EEF-2 quantified in Fig. S2J; for full membrane see Fig. S2I. E WB of eEF2 T56 phosphorylation (p-eEF2), eEF2, tubulin, and eEF2K in A549 lung cancer cells in fed condition and after serum/glucose deprivation for 3, 6, and 24 h. N = 4; p-eEF2 quantified in Fig. S2K; for full membrane see Fig. S2L. WT, wild type.

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