Fig. 4: efk-1 promotes starvation survival independently of its kinase activity and of EEF-2 T56 phosphorylation.

A RT-qPCR of efk-1 mRNA expression in WT, efk-1(ok3609) null mutants, and two lines of efk-1 kinase-dead mutants, efk-1(ste2) and efk-1(ste3), referred to as efk-1(D257A) line 1 and line 2 respectively. N = 3, error bars represent mean ± SD; ***p = 0.0008 (one-way ANOVA with Tukey’s multiple comparisons test). B WB of p-EEF-2, EEF-2, and tubulin in fed L4 larvae of WT, efk-1 null mutants, and two lines of efk-1 kinase-dead mutants as described in A. N = 3; for whole membrane images, see Fig. S4B. C The graph shows L1 starvation survival of WT, efk-1(ok3609), and two lines of efk-1 kinase-dead mutants described in A. N = 3, error bars represent mean ± SD; **p = 0.002 percent L4 vs. WT animals (AUC compared using one-way ANOVA with Tukey’s multiple comparisons test). D WB of p-EEF-2, EEF-2, and tubulin in fed day-one adults of WT and eef-2(ste1) mutants bearing an EEF-2 T56A phosphosite mutation, denoted as eef-2(T56A). N = 3; for whole membrane images, see Fig. S4D. E The graph shows L1 starvation survival of WT and eef-2 phosphosite-dead mutants as described in D. N = 3, error bars represent mean ± SD; percent L4 vs. WT animals (AUC compared using two-tailed unpaired t-test). WT, wild type; ns, not significant; AUC, area under the curve. Source data are provided as a Source Data file.