Fig. 3: Cellular clonal proliferation contributes to proviral expansion. | Nature Communications

Fig. 3: Cellular clonal proliferation contributes to proviral expansion.

From: Barcoded HIV-1 reveals viral persistence driven by clonal proliferation and distinct epigenetic patterns

Fig. 3

a Schematic depicting proliferation of infected cell lineages measured by UMI per IS. b Contribution of proliferated and non-proliferated cells among all infected cells harboring provirus. c Contribution of proliferated cell clones harboring provirus by timepoint. d Contribution of proliferated and non-proliferated cells among all infected cells harboring provirus. e Cell clone size (UMI per IS) for each proliferated cell clone by timepoint. Horizontal bars represent mean values. n.s., p > 0.05. P value was calculated using the two-tailed Mann-Whitney test. n represents the number of IS. f–h Correlation of natural log transformed proviral barcode frequency and UMI per IS per sample for each timepoint among the proviral barcodes in proliferated cell clones during early infection (f), ART suppression (g), or rebound infection (h). Black trendline line calculated by simple linear regression. Spearman’s rank correlation coefficient (r) and associated p values. Early, n = 88; ART, n = 272; Rebound, n = 144. Source Data b–h.

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