Fig. 5: Translational landscape of viral transcripts. | Nature Communications

Fig. 5: Translational landscape of viral transcripts.

From: Non-AUG HIV-1 uORF translation elicits specific T cell immune response and regulates viral transcript expression

Fig. 5

A HIV-1 genomic structure. B Distribution of RNA-seq (red) and ribosome profiling (blue) reads across the HIV-1 genome in SupT1 cells at 1, 12, 24 and 36 hpi (n = 4 independent experiments). C Translation efficiency of the genomic RNA (across both Gag and Pol coding sequences) at each time point of infection (n = 4 independent experiments). Boxplots are defined as minima, 1st quartile, median, 3th quartile and maxima. D Translation of incoming viral genomic RNAs as tested by infecting cells pre-incubated or not with cycloheximide or puromycin with a recombinant replication-competent HIV-1 virus bearing a GFP sequence within Gag (n = 4 independent experiments). Barplots correspond to the mean value of all biological replicates. A one tailed, paired t test was performed to compare samples. Source data are provided as a Source Data file (Partially created in BioRender. Ricci, E. (2022) BioRender.com/k47z556 and using an Illustration from NIAID NIH BIOART Source https://bioart.niaid.nih.gov/bioart/160). E Translation efficiency of canonical viral mRNAs, 12, 24 and 36 hpi (n = 4 independent experiments). Points correspond to the mean value of all biological replicates and error bars correspond to the Standard Error of the Mean (SEM). F Percentage of Gag-Pol ribosome frameshifting at each time point of infection (n = 4 independent experiments). Boxplots are defined as minima, 1st quartile, median, 3th quartile and maxima.

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