Fig. 7: Non-AUG translation initiation sites in the 5’UTR of viral transcripts. | Nature Communications

Fig. 7: Non-AUG translation initiation sites in the 5’UTR of viral transcripts.

From: Non-AUG HIV-1 uORF translation elicits specific T cell immune response and regulates viral transcript expression

Fig. 7

A (Top panel) Distribution of ribosome profiling reads across the HIV-1 genome from infected U937 (Green, n = 3 independent experiments), SupT1 (Red, n = 4 independent experiments) and primary CD4 + T lymphocytes (Blue, n = 2 independent experiments). All y axis correspond to reads per million values - RPM. (Bottom panel) Close-up view of the unspliced HIV-1 5’UTR showing the distribution of ribosome profiling reads and the position of putative non-AUG start codons as well as the open-reading frames of putative peptides produced from non-AUG start codons. The y-axis corresponds to reads per million of sequenced reads (RPM). B Distribution of FLOSS (Fragment Length Distribution Score) values for cellular and viral transcripts computed from ribosome profiling reads from SupT1 (left panel), U937 (middle panel) or primary CD4 T cells (right panel).

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