Fig. 3: Detection of T cell responses specific to ARF-derived peptides (ARFP) in PBMCs of PLWH.
From: Unveiling conserved HIV-1 open reading frames encoding T cell antigens using ribosome profiling
a Overview of ARFP-specific T cell responses. The number of ARFP-specific T cell responses is represented for each donor. The largest rectangle represents different peptides encoded by the same ARF. The color code, on the top right corner, indicates the overlapping CDS. b Number of responding PLWH to each ARFP stimulation. As in (a) the color code indicates the overlapping CDS. The rough data for IFNγ-Elispot responses are presented in Supplementary Fig. 7-related to Fig. 3. c IFNγ-ELISPOT deconvolution of positive pools at day 12 and for all tested donors. Only IFNγ-positive T cell responses upon individual ARFP stimulations are presented, in Spot Forming unit (SFU)/106 PBMCs. Intrinsic negative controls (POOL (−), cells incubated with DMSO-containing medium) are indicated by white bars for each tested pool and the respective peptides. POOL (+), HIV (+), and CEF (+) correspond to PBMCs loaded with the pool of ARF-derived, HIV-classical, and non-HIV common virus-derived peptides, respectively. The color code indicates the overlapping CDS. SAT: saturated well for which the IFNγ signal was too high to be quantified. Data are presented as mean values of technical triplicates ± SD. d Genomic repartition of ARF encoding immunogenic peptides. The number of ARFs is indicated in brackets. The color code is as in (a).
