Fig. 2: Defective nucleosome binding of BAF and Lamin A/C IgF mutants.

a Schematic representation of the pull-down assay with the biotinylated nucleosome. b BAF and Lamin A/C IgF (or Lamin A/C IgF K486N or K486A mutant) were incubated with the biotinylated nucleosome and captured with streptavidin-magnetic beads. Proteins eluted from the streptavidin-magnetic beads were analyzed by SDS-PAGE with Coomassie Brilliant Blue (CBB) staining. c, d Quantification of the nucleosome binding by BAF and Lamin A/C IgF in panel (b). The band intensities of Lamin A/C IgF mutants (c) and BAF (d) (lanes 10 and 11) were normalized to those in lane 9. Bar graphs and circles show the means and individual values of three independent experiments, respectively. Error bars represent the standard deviation. e BAF and Lamin A/C IgF (or Lamin A/C IgF H506D or H506A mutant) were incubated with the biotinylated nucleosome and captured with streptavidin-magnetic beads. Proteins eluted from the streptavidin-magnetic beads were analyzed by SDS-PAGE with CBB staining. f, g Quantification of the nucleosome binding by BAF and Lamin A/C IgF in panel (d). The band intensities of Lamin A/C IgF mutants (f) and BAF (g) (lanes 10 and 11) were normalized to those in lane 9. Bar graphs and circles show the means and individual values of three independent experiments, respectively. Error bars represent the standard deviation. h BAF (or BAF S4E) and Lamin A/C IgF were incubated with the biotinylated nucleosome and captured with streptavidin-magnetic beads. Proteins eluted from the streptavidin-magnetic beads were analyzed by SDS-PAGE with CBB staining. i, j Quantification of the nucleosome binding by BAF and Lamin A/C IgF in panel (h). The band intensities of Lamin A/C IgF (i) and the BAF mutant (j) (lanes 8 and 10) were normalized to those in lane 6. Bar graphs and circles show the means and individual values of three independent experiments, respectively. Error bars represent the standard deviation. Source data are provided as a Source Data file.