Table 3 Plasmids used in this study
Plasmid name | Relevant genotype | Antibiotic resistance used for selection | Ori / backbone | Reference |
|---|---|---|---|---|
pKD3 | FRT-cat-FRT | Cm | ||
pKD4 | FRT-aphT-FRT | Kan | ||
pKD46 | mScarlet counter-selection cassette | Amp | ||
pCP20 | FLP recombinase | Amp, Cm | ||
pZ8001 | PglpT-gfpmut2 | Amp | pWKS30/pSC101 | This study |
pM3101 | promoterless-gfpmut2 | Amp | pSC101 | |
pZ8049 | PpstS-nanoluc | Amp | pSC101 | This study |
pZ8046 | PgloC-nanoluc | Amp | pSC101 | This study |
pZ8040 | promoterless-nanoluc | Amp | pSC101 | This study |
pZ8044 | PrpsM-nanoluc | Amp | pSC101 | This study |
pZ8026 | Pnat-glpT | Amp | pSC101 | This study |
pZ7903 | Empty vector | Amp | pSC101 | Kindly provided by Thea Bill Andersen (manuscript in preparation) |
