Fig. 2: Multiple modes of oligo subset selection were enabled by synthesis and selection along with a hierarchical barcode system.

a Five oligos of varying lengths with identical universal primers were designed and mixed at equal molar concentrations before selection. A 4 nt barcode was assigned to demonstrate various selection methods. b Single, (c) hierarchical, and (d) multiple selections of oligo subsets were demonstrated. The hierarchical structure of the barcodes for the five oligos is depicted on the left, where selecting a barcode at a higher level enables simultaneous selection of all connected lower-level barcodes, akin to accessing files within a folder. Barcodes highlighted in cyan are examples used for selection, with the corresponding selected oligos illustrated next to them. The results of polyacrylamide gel electrophoresis after selection are displayed, listing the selected barcodes in sequence below the image. These experiments (b, c, d) were repeated three times independently with similar results.