Fig. 4: Selection efficiency of multiple cycles of selection and rare population of oligo subset.

a The read count ratio for every synthesis and selection cycle of 4 nt barcodes was analyzed using 10 distinct barcodes. The bars represent one standard deviation from the average in this figure (n  =  10 sequencing reads). b The Enrichment fold (EF) values of the target oligos were calculated after aligning the barcode and inserting the sequence to the reference sequence. Each barcode of aligned sequences showed no significant bias within EF value; the dotted line represents the average of each EF. c The ratio of selected target sequences by sequencing coverage (n  =  10 sequencing reads). Results are compared with PCR-based selection. d Demonstration of subset selection within the rare population of an oligo library. The read count ratios for each cycle of four distinct 8 nt barcode selections were analyzed. The blue graph illustrates the ratio for one barcode assigned to three oligo designs, and the green graph illustrates the ratio for three barcodes each assigned to one designed strand, out of a diversity of 12,000. e The distribution of read counts per million of all aligned sequences were analyzed. The left graph shows the barcode number distribution before selection, and the right graph shows the distribution after selection. The ratio of target oligos were increased by an average of 57.86 times following the selection process.