Fig. 5: Demonstrating subset replacement through negative synthesis and selection in a hierarchically structured oligo library.
From: Oligonucleotide subsets selection by single nucleotide resolution barcode identification
a Each layer of the barcode sequences corresponds to different levels of data, from musical pieces to specific instrument sections. The barcode ‘ACTG’ targets a segment of Pachelbel’s Canon in D Major. The process represents the replacement from the original data (contrabass #5) with new data (violin #5) through negative synthesis and selection. The sheets of music are the results of converting the original MIDI file of the music into an SVG file (contrabass #5: https://musescore.com/user/94946395/scores/23072350?share=copy_link, violin #5: https://musescore.com/user/94946395/scores/23072362?share=copy_link) using the MuseScore app (https://musescore.org/ko). The MIDI file is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 license. b The negative synthesis and selection process followed by file replacement involves blocking the target barcode while allowing the selection of the others. Subsequently, a newly synthesized oligo subset is added to replace the file. c To verify the target file replacement, we plotted the normalized read counts from various barcode selection scenarios in a bar graph.
