Fig. 3: FH11 localizes at the plasma membrane and promotes polymerization of actin filaments. | Nature Communications

Fig. 3: FH11 localizes at the plasma membrane and promotes polymerization of actin filaments.

From: Control of plasma membrane-associated actin polymerization specifies the pattern of the cell wall in xylem vessels

Fig. 3

a Diagram of FH11 protein showing truncated fragments. SP, EC and TM indicate the signal peptide, extra-cytoplasmic Ser/Pro rich, and transmembrane domains, respectively. Numbers indicate the locations of amino acid residues relative to the full-length protein. b and c FH11 (LexApro:FH11-EYFP) and plasma membrane (FM4-64) in Arabidopsis cells cultured without (b) or with (c) 400 mM mannitol. In (b), the intensity profiles along the white lines (indicated by white arrowheads) are shown in graphs to the right of each panel. The white box in (c) shows Hechtian strands induced by 400 mM mannitol; an enlarged image of this area is shown in the far-right panel. The images are representative of three independent experiments. All scale bars = 20 µm, except for the enlarged image, in which scale bar = 10 µm. d Actin filaments (UBQ10pro:Lifeact-mGFP) in root epidermal cells harboring LexApro:FH11-tagRFP treated with (+Est) or without (-Est) 2 µM estradiol. Arrows indicate actin cables. The lower panels show xz slices along the white lines. Scale bars = 5 µm [xy] or 2.5 µm [xz]. e Skewness and density of actin filaments. Dots are individual data points; the horizontal line indicates the median; n = 51 (-Est) and 52 (+Est) cells; ****: P < 0.0001 (two-tailed Student’s t-test). f Histogram of fluorescent intensity along the white lines shown in (d). Mean values ± standard deviations are Control: 1636.8 ± 2243.3; FH11 (full): 840.8 ± 220.8, (two-tailed Student’s t-test, P = 1.20E-6).

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