Fig. 2: Broad spectrum activity of P33 peptides.

A Distribution of the BLOSUM80 scores of homologous sequences matching the P33 APR across the E. coli proteome, compared to the distribution obtained with 10 random peptides of the same length. B Fraction of scores in its distribution corresponding to high similarity scores (BLOSUM80 score > 60% of the self-match score). The data are represented as mean ± S.D., student t-test was used for statistical analysis. C Number of high similarities matches of the P33 APR in the full proteome panels of ESKAPE pathogens. The plot represents the exact number of matches with the indicated properties. D Minimum inhibitory concertation (MIC) value of P33 against 132 clinical isolates from six different bacterial species. The plot shows all the data points, as well as the mean and standard deviation. E, F Effect of P33 peptides on biofilm formation in strains of S. aureus, E. coli, and P. aeruginosa. Data represent the average and standard deviation of three independent biological replicates (n = 3), each derived from different experimental units (separate bacterial cultures). The unit of study is the independent bacterial culture, which was assigned a specific treatment condition. Error bars represent the standard deviation across these biological replicates. Control groups, where applicable, are explicitly defined as the buffer-treated samples.