Fig. 4: Fast and slow skeletal muscle fiber signatures.
From: Human skeletal muscle fiber heterogeneity beyond myosin heavy chains
A, B Volcano plots comparing the slow and fast clusters identified from the uniform manifold approximation and projection (UMAP) plots in Fig. 1G–H. Colored dots represent significantly different transcripts or proteins at an FDR < 0.05, darker dots represent significantly different transcripts or proteins with a log fold change > 1. Two-sided statistical analysis was performed with the DESeq2 Wald test with Benjamini-Hochberg adjusted p-values (transcriptomics) or a Limma linear model approach with empirical Bayes methods followed by p-value adjustment for multiple comparisons using the Benjamini-Hochberg method (proteomics). C Feature plots of selected differentially expressed genes or proteins between slow and fast fibers. D Enrichment analysis of significantly different transcript and proteins. Overlap – enriched in both datasets, Transcriptome – enriched only in transcriptome, Proteome – enriched only in proteome. Statistical analysis was performed using the clusterProfiler package with Benjamini-Hochberg adjusted p-values. E Fiber type specific transcription factors as identified by SCENIC, based on SCENIC-derived regulon specificity score and differential mRNA expression between fiber types. F Feature plots of selected differentially expressed transcription factors between slow and fast fibers.
