Fig. 4: VDAC2-dependent sensitivity to tBid in hepatocytes and HCC cells.
A Representative time course measurements of ΔΨm in tBid-treated, permeabilized rat hepatocytes infected with adenovirus encoding VDAC2 or GFP (left) and dot plot showing the changes in TMRE fluorescence during the treatment period (n = 4 experiments with 1 or 2 technical replicates per experiment; p = 0.0039, two-tailed t-test). B As in (A) but treatment with 75 nM Bax instead of tBid; n = 2 experiments. C Immunoblot of cyto c in the supernatant fraction from ΔΨm measurements in (A) and (B). D Representative immunoblots of VDAC2 and Bak in membrane fractions and (E) Bax in whole cell lysates of rat hepatocytes infected with Ad-VDAC2 and Ad-GFP. Prohibitin and Actin used as loading controls. VDAC2 blot is cropped between the 25 and 37 kDa molecular weight markers. Experiments were repeated 4 times with similar results. F Representative time course measurements of ΔΨm in permeabilized tBid-treated WT and VDAC2 knockout HepG2 cells. The experiment was repeated twice with similar results G Time course measurements of ΔΨm in permeabilized tBid-treated WT and VDAC2 knockout PLC/PRF/5 cells. The experiment was repeated 4 times with similar results. Source data are provided in the Source Data file.
