Fig. 5: Structural rearrangements of LAT1 for substrate transport.
From: Structural basis of anticancer drug recognition and amino acid transport by LAT1
a Superposition of three major conformational states of LAT1 observed in the study, depicting the rocking-bundle movements relevant to substrate translocation. The hash domain of LAT1 is colored gray and TM1’ of CD98hc is green. The bundle domain is colored differently for three conformations: inward-open (pink), occluded (purple) and outward-open (cyan). b, c Superposition of TM10 in the three conformations. d Superposition of TM12 in the three conformations. e Close-up view of the N-terminal region of LAT1 in the outward-open state. The cryo-EM density map for the region is shown as blue mesh, contoured at 0.06. f Uptake of ʟ-[14C]Leu into Xenopus oocytes expressing CD98hc and wild-type or Δ1–50 LAT1. Data are mean ± SD and each data point represents a single oocyte (n = 8 for WT; n = 9 water; and n = 10 for CD98hc and Δ1–50). Source data are available with this paper as Source Data file.
