Fig. 7: Depletion of PAFAH1B1, RPS3A, SMU1 and VPS16 suppress the larvae lethality of an NGPS hermaphrodite C. elegans model.
From: A multiparametric anti-aging CRISPR screen uncovers a role for BAF in protein synthesis regulation
A Confocal images of hypodermal nuclei of live wild-type (WT; strain COP262) and NGPS (G12T; strain BN1389) 1 day old adult hermaphrodites C. elegans expressing FIB-1::GFP. Shown are overlays of GFP (green) and DIC images. Scale bar 5 um. B Nucleolar area was measured in 124 nucleoli for the WT worms and 146 nucleoli for the baf-1(G12T), representing > 20 animals per strain over 4 independent experiments. Black lines show the average nucleolar area ± SD. The p-value from Welch two sample t-test is indicated. C The indicated genes were knocked down by RNAi and tested for suppression of lethality in gfp::lmn-1, baf-1(G12T) hermaphrodites (strain BN1336). Each point corresponds to the percentage of eggs developing into larvae from a single plate with 50-100 eggs laid by 1 day old adult hermaphrodites. For PAFAH1B1, 9 plates were analyzed for both the control and PAFAH1B1, over 3 independent experiments. For RPS3A, 15 control plates and 14 RPS3A plates were analyzed over 4 independent experiments. For SMU1, 14 control and 16 SMU-1 plates were analyzed over 5 independent experiments. For VPS16, 12 controls and 11 VPS16 plates were analyzed over 4 independent experiments. Black lines indicate medians. Mann-Whitney test p-values were calculated for each set of control and test plates.
