Fig. 1: Cross-species correlation of EDA2R expression with age and upregulation in premature aging models. | Nature Communications

Fig. 1: Cross-species correlation of EDA2R expression with age and upregulation in premature aging models.

From: Increased ectodysplasin-A2-receptor EDA2R is a ubiquitous hallmark of aging and mediates parainflammatory responses

Fig. 1

a Boxplots representing average Spearman’s correlation coefficients between mRNA expression of all genes with age, from the GTEX dataset. Correlations were calculated across 10-fold Leave-Half-Out random resampling per tissue. Relative rankings of EDA2R, EDAR, and EDA are indicated. Sample sizes for each tissue stratified by sex are shown at the top. b Representation of the mean Spearman’s correlation coefficients (ρ) for each gene across solid tissues, with red and blue dots indicating genes that increase or decrease with age, respectively. c Boxplots representing the median Pearson’s correlation coefficients between age and mRNA expression across tissues of mice (lightgrey) and rats (darkgrey), as determined from Tabula Muris Senis (GSE132040 dataset, 14 tissues, n = 850 distinct samples) and Rat BodyMap (GSE53960 dataset, 11 tissues, n = 320 distinct samples). Mouse and rat icons: Created in BioRender. Bolis, M. (2025) https://BioRender.com/h42n006. d Left, Volcano-plot representing differential expression in murine aortic artery of HGPS, 2 months old mice (LMNA-G609G mutant, n = 6) versus wild-type, 2 months old age-matched mice (GSE165409, n = 6). Blue and red colors indicate down or upregulated genes with a log2 fold-change lower than -1 or greater than 1 and an FDR < 0.05. Right, Boxplots representing Eda2r expression in the aortic artery of HGPS (pink, n = 6) and wild-type (green, n = 6) mice. P-values were computed using Wald statistics (two-sided) as implemented in DESeq2, with p-values adjusted for multiple comparisons using the Benjamini-Hochberg procedure. Indicated is the exact FDR of Eda2r q = 5.8e−70. Mouse icon: Created in BioRender. Bolis, M. (2025) https://BioRender.com/i32c095. e Left, boxplots comparing microarray expression levels of Eda2r between HPGS (pink) and age-matched wild-type (green) mice in murine keratinocytes (GSE67288, n = 8 biological replicates in each group) and (right) gonadal adipocytes (GSE51203, n = 3 biological replicates in each group). Mutant mice deficient of Lamin C but not Lamin A (LCS, lightgreen) are provided as comparison. P-values were calculated using the Wilcoxon Rank-Sum test (two-sided) and adjusted for multiple comparisons using FDR. Mouse icon: Created in BioRender. Bolis, M. (2025) https://BioRender.com/m99g202. – Boxplots boundaries and source data are provided as a Source Data file.

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