Fig. 1: The anatomy of M. amalyticus predation of G. amarae.
a Example tomogram of G. amarae CON44T and M. amalyticus interaction, images are 2D slices through a 3D tomogram (weighted back projection, TomoSim filtered, thickness = 10). M. amalyticus cell (~300–500 nm in length) can be seen forming a tight interaction with G. amarae CON44T cell (~500–1000 nm in width). b A closer view of the interaction reveals a tube density forming between the two cells (pink arrow head), and protein array forming within G. amarae (green arrow head). c, d Segmented 3D volumes of tomograms shown in (a, b) (pili = purple; lance/tube structure = pink; G. amarae cytoplasmic membrane = green; cytoplasmic vesicles = yellow; storage granule = orange; M. amalyticus JR1 outer layer = dark blue; M. amalyticus cytoplasmic membrane = teal). e Example tomograms of M. amalyticus JR1, images are 2D slices (thickness = 10) through 3D reconstructed tomogram. Cell membranes, type 4 pili and a lance-like structure are clearly visible. f Closer view of the lance structure protruding from the M. amalyticus cell, parallel lines through the cell envelope of M. amalyticus are indicative of a lance/tube structure. g Cellular compartments present at the poles of predatory M. amalyticus cells during attachment to a G. amarae cell, closer view shown in the inset. All scale bars represent 50 nm (See supplementary movie 1 for more detail).
