Fig. 5: Functional differences between the TERT-FL and TERT-β isoforms. | Nature Communications

Fig. 5: Functional differences between the TERT-FL and TERT-β isoforms.

From: Genetic regulation of TERT splicing affects cancer risk by altering cellular longevity and replicative potential

Fig. 5

a Structured illumination microscopy images of A549 cells co-transfected with TERT-FL and TERT-β expression constructs at a 50:50% ratio. For individual channels, the staining is shown as black/white images for better contrast. Tri-color merged panels: green – FLAG (TERT-β) or HA (TERT-FL), blue – DAPI (nuclei). Quad-color merged panel: purple - HA (TERT-FL), green - FLAG (TERT-β), red - TOM20 (mitochondria), blue - DAPI (nuclei). The yellow inset in the TERT-β-FLAG panel is shown at a higher magnification to demonstrate colocalization with mitochondria (yellow staining). The images shown are representative of ten distinct images taken across two independent experiments. Scale bar = 2 μm, inset scale bar = 0.5 μm. b Schematic overview of the proposed relationships between VNTR6-1 repeat length, the TERT-FL:TERT-β ratio, proliferation, and apoptosis.

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