Fig. 2: TMH-1 as a key region regulating the conformation of the HAS transmembrane channel. | Nature Communications

Fig. 2: TMH-1 as a key region regulating the conformation of the HAS transmembrane channel.

From: Regulating cellular metabolism and morphology to achieve high-yield synthesis of hyaluronan with controllable molecular weights

Fig. 2

A, B Comparative analysis of the tertiary structure and amino acid sequences between SpHAS and SzHAS. C Analysis of HA synthesis and transport in C. glutamicum expressing SzHAS mutants: SzHASSpTMH-1, SzHASSpTMH-2, SzHASSpTMH-3, SzHASSpTMH-4, SzHASSpIF-1, SzHASSpIF-2, and SzHASSpIF-3. D PH, bright field (BF), and TEM micrographs of C. glutamicum cells expressing SzHASSpTMH-1. E Growth curve, glucose consumption, and HA production of C. glutamicum expressing SpHAS mutant SpHASSzTMH-1. F PH, BF, and TEM micrographs of C. glutamicum cells expressing SpHASSzTMH-1. G Protein structure of SzHAS predicted by AlphaFold2. H Western blotting (WB) analysis showing expression of HAS mutants SzHASΔTMH-1 and SpHASΔTMH-1. HA synthesis by HAS mutants was analyzed in C. glutamicum. BF microscopy shows cells under BF after negative staining with 100 mg mL−1 nigrosin for 5 min. All data presented as mean ± S.D. from three (n = 3) biological independent replicates. All microscopy experiments were performed in triplicate with similar results. Source data are provided as a Source Data file.

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