Fig. 2: Content of S100A12 significantly increased in the myocardial tissue of TG mice during the early stages after MI, in parallel to leukocyte infiltration and neutrophil extracellular traps (NETs) formation.

a, b Representative immunohistochemical (IHC) staining of S100A12 and quantification in the border area of infarcted myocardial tissue of TG mice at different time points after MI (n = 4 biological replicates). Scale bar, 50 μm. c S100A12 mRNA expression level was analyzed in the border area of TG mice at different time points after MI (n = 6 biological replicates). d, e Representative blots of S100A12 and quantitative analysis in the border area of TG mice at different time points after MI (n = 4 biological replicates). f–h Immunofluorescence double staining of neutrophil marker (Ly6G) and S100A12 in the border area of TG and WT mice and quantification at day 1 post-MI (n = 4 biological replicates). Scale bar, 20 μm. i, j Representative blots of NETs-related proteins [myeloperoxidase (MPO), neutrophil elastase (NE), and citrullination of histone 3 (citH3)] and quantification in WT and TG mice at Day 1 after MI or sham operation. One-way ANOVA with Tukey’s multiple comparisons test is used for statistical analyses of MPO, Brown-Forsythe and Welch’s ANOVA test is used for analyses of NE and citH3 (n = 4 biological replicates). k–n Representative IHC staining of NET-related proteins and quantification in WT and TG mice at Day 1 post-MI (n = 4 biological replicates). Scale bar, 50 μm. Data are expressed as mean ± SEM. Statistical analyses were carried out using one-way ANOVA with Tukey’s multiple comparisons test for (h), (l), (m), (n) and Brown-Forsythe and Welch’s ANOVA test for (b), (c), (e), (g). Source data are provided as a Source Data file.