Fig. 6: Experimental design outlining the timeline for each cohort. | Nature Communications

Fig. 6: Experimental design outlining the timeline for each cohort.

From: Bacteria invade the brain following intracortical microelectrode implantation, inducing gut-brain axis disruption and contributing to reduced microelectrode performance

Fig. 6

A The unimplanted mice were sacrificed two weeks after housing separation for analysis. The 4- and 12-week post-implantation animals undergo implantation, fecal collection, neural recordings, and perfusion at their endpoint. B The 4-week cohort received four non-functional dummy implants and the 12-week cohort received one non-functional dummy implant and one functional intracortical microelectrode (IME) implant with respective ground and reference wires. C 12-week functional implanted mice were recorded using a commutator hooked up to the TDT LabRat Ephys system. D 16S analysis was done on a biopsy of brain tissue around the implant site and on fresh fecal matter collected from each animal. E Cell-specific spatial proteomics and spatial transcriptomics were performed on various brain samples sectioned onto microscope slides. Created in BioRender. Capadona, J. (2025) https://BioRender.com/p93j360.

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